A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide.
Identifieur interne : 001D71 ( Main/Exploration ); précédent : 001D70; suivant : 001D72A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide.
Auteurs : Changhyun Roh [Corée du Sud]Source :
- International journal of nanomedicine [ 1178-2013 ] ; 2012.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (génétique), Analyse par réseau de protéines, Antiviraux (), Antiviraux (pharmacologie), Boîtes quantiques, Catéchine (analogues et dérivés), Catéchine (pharmacologie), Conformation d'acide nucléique, Nanomédecine, Nanoparticules, Oligoribonucléotides (), Oligoribonucléotides (génétique), Protéines nucléocapside (antagonistes et inhibiteurs), Protéines nucléocapside (génétique), Séquence nucléotidique, Tests de sensibilité microbienne (), Virus du SRAS (), Virus du SRAS (génétique).
- MESH :
- analogues et dérivés : Catéchine.
- antagonistes et inhibiteurs : Protéines nucléocapside.
- génétique : ARN viral, Oligoribonucléotides, Protéines nucléocapside, Virus du SRAS.
- pharmacologie : Antiviraux, Catéchine.
- ARN viral, Analyse par réseau de protéines, Antiviraux, Boîtes quantiques, Conformation d'acide nucléique, Nanomédecine, Nanoparticules, Oligoribonucléotides, Séquence nucléotidique, Tests de sensibilité microbienne, Virus du SRAS.
English descriptors
- KwdEn :
- Antiviral Agents (chemistry), Antiviral Agents (pharmacology), Base Sequence, Catechin (analogs & derivatives), Catechin (pharmacology), Microbial Sensitivity Tests (methods), Nanomedicine, Nanoparticles, Nucleic Acid Conformation, Nucleocapsid Proteins (antagonists & inhibitors), Nucleocapsid Proteins (genetics), Oligoribonucleotides (chemistry), Oligoribonucleotides (genetics), Protein Array Analysis, Quantum Dots, RNA, Viral (chemistry), RNA, Viral (genetics), SARS Virus (drug effects), SARS Virus (genetics).
- MESH :
- chemical , analogs & derivatives : Catechin.
- chemical , antagonists & inhibitors : Nucleocapsid Proteins.
- chemical , chemistry : Antiviral Agents, Oligoribonucleotides, RNA, Viral.
- chemical , genetics : Nucleocapsid Proteins, Oligoribonucleotides, RNA, Viral.
- chemical , pharmacology : Antiviral Agents, Catechin.
- drug effects : SARS Virus.
- genetics : SARS Virus.
- methods : Microbial Sensitivity Tests.
- Base Sequence, Nanomedicine, Nanoparticles, Nucleic Acid Conformation, Protein Array Analysis, Quantum Dots.
Abstract
Hundreds of million people worldwide have been infected with severe acute respiratory syndrome (SARS), and the rate of global death from SARS has remarkably increased. Hence, the development of efficient drug treatments for the biological effects of SARS is highly needed. We have previously shown that quantum dots (QDs)-conjugated RNA oligonucleotide is sensitive to the specific recognition of the SARS-associated coronavirus (SARS-CoV) nucleocapsid (N) protein. In this study, we found that a designed biochip could analyze inhibitors of the SARS-CoV N protein using nanoparticle-based RNA oligonucleotide. Among the polyphenolic compounds examined, (-)-catechin gallate and (-)-gallocatechin gallate demonstrated a remarkable inhibition activity on SARS-CoV N protein. (-)-catechin gallate and (-)-gallocatechin gallate attenuated the binding affinity in a concentrated manner as evidenced by QDs-conjugated RNA oligonucleotide on a designed biochip. At a concentration of 0.05 μg mL(-1), (-)-catechin gallate and (-)-gallocatechin gallate showed more than 40% inhibition activity on a nanoparticle-based RNA oligonucleotide biochip system.
DOI: 10.2147/IJN.S31379
PubMed: 22619553
Affiliations:
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Le document en format XML
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nucléique</term><term>Nanomédecine</term><term>Nanoparticules</term><term>Oligoribonucléotides ()</term><term>Oligoribonucléotides (génétique)</term><term>Protéines nucléocapside (antagonistes et inhibiteurs)</term><term>Protéines nucléocapside (génétique)</term><term>Séquence nucléotidique</term><term>Tests de sensibilité microbienne ()</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Catechin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antiviral Agents</term><term>Oligoribonucleotides</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Oligoribonucleotides</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Catechin</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Catéchine</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Oligoribonucléotides</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microbial Sensitivity Tests</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term><term>Catéchine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Nanomedicine</term><term>Nanoparticles</term><term>Nucleic Acid Conformation</term><term>Protein Array Analysis</term><term>Quantum Dots</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Analyse par réseau de protéines</term><term>Antiviraux</term><term>Boîtes quantiques</term><term>Conformation d'acide nucléique</term><term>Nanomédecine</term><term>Nanoparticules</term><term>Oligoribonucléotides</term><term>Séquence nucléotidique</term><term>Tests de sensibilité microbienne</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hundreds of million people worldwide have been infected with severe acute respiratory syndrome (SARS), and the rate of global death from SARS has remarkably increased. Hence, the development of efficient drug treatments for the biological effects of SARS is highly needed. We have previously shown that quantum dots (QDs)-conjugated RNA oligonucleotide is sensitive to the specific recognition of the SARS-associated coronavirus (SARS-CoV) nucleocapsid (N) protein. In this study, we found that a designed biochip could analyze inhibitors of the SARS-CoV N protein using nanoparticle-based RNA oligonucleotide. Among the polyphenolic compounds examined, (-)-catechin gallate and (-)-gallocatechin gallate demonstrated a remarkable inhibition activity on SARS-CoV N protein. (-)-catechin gallate and (-)-gallocatechin gallate attenuated the binding affinity in a concentrated manner as evidenced by QDs-conjugated RNA oligonucleotide on a designed biochip. At a concentration of 0.05 μg mL(-1), (-)-catechin gallate and (-)-gallocatechin gallate showed more than 40% inhibition activity on a nanoparticle-based RNA oligonucleotide biochip system.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Roh, Changhyun" sort="Roh, Changhyun" uniqKey="Roh C" first="Changhyun" last="Roh">Changhyun Roh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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